Cytoplasmic and Nuclear Protein Extraction Kit
This kit supplies a complete set of lysis reagents that enable the separation of nuclear protein and cytoplasmic fractions from cultured cells and tissue. The optimized reagents and protocol allow non-denatured, active proteins to be purified in less than two hours. It is sufficient for extracting 50-100 protein samples, the quantity for each sample is (5-10) x 108 cells (about 50mg).
Features
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Obtain purified protein in 90 minutes.
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No ultracentrifugation is required.
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Cross-contamination of cytosolic proteins into the membrane fractions is about 10%
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ready-to-use in downstream applications such as NGS, RT-PCR, and qPCR, northern blot, polyA screening, etc
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Wen HC, Huo YN, Chou CM, Lee WS. PMA inhibits endothelial cell migration through activating the PKC-δ/Syk/NF-κB-mediated up-regulation of Thy-1. Sci Rep. 2018 Nov 2;8(1):16247.
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Hsu SH, Chou LF, Hong CH, Chang MY, Tsai CY, Tian YC, Yang HY, Yang CW. Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis. Int J Mol Sci. 2021 Dec 4;22(23):13132.
Q1. Do you need to add protease, phosphatase inhibitor in every reagent?
A: Yes. This is the optimal step.
A: Yes. This is the optimal step.